BIO380 - Biochemistry

FALL 2012

Location and Meeting Time:  Wold Center 225 MWF 9:15AM-10:20AM
Laboratory: WOLD 201,  WEDNESDAY-THURSDAY  1:45-4:35 PM



The main objectives of this course are to understand some of the fundamental concepts involved in the underlying biochemistry of a cell, and to learn how to think critically.  Emphasis will be placed on describing the functions and structure of lipids, nucleotides, and carbohydrates.  These include the role of lipids in cell membranes, the non-nucleic acid functions of nucleotides, and the role of glycosylation in protein function.  We will try and understand how structure is integrated with function, and the means by which the cell carries out these important processes.

  1. TEXTBOOKS (required)

    Fundamentals of Biochemistry, Third Edition by Donald Voet, Judith G. Voet and Charlotte W. Pratt (2008), published by John Wiley & Sons (ISBN: 978-0-470-12930-2). This textbook is required, as there will be assigned readings (please see lecture schedule) that will either complement or expand upon the material presented during lectures.  However, the lecture period remains the most important and efficient way of learning the material in the course.

    The Double Helix by James Watson (1968 and 1996), published by Touchstone (Simon and Schuster) (ISBN for paperback version: 074321630X).


    Lectures will be given in the Wold Center Room 225 every Monday, Wednesday and Friday between 9:15 AM and 10:20 AM.  Presentations will make use of traditional teaching tools as well as assorted electronic resources.  I will rely heavily on Nexus to transmit files including supplemental readings and slides presented in class.  If you have any problems please let me know so I can find another way to get you the required files.


    Students enrolled in the course will be required to attend lab once each week.  Labs will be held in the Wold Center Room 201 WEDNESDAY AND THURSDAY.  If you cannot make your scheduled lab period a particular week, you must make it up that same week in another period.  Failure to do so will result in a failing grade for the lab section of the course.

    1. Lecture-based term-tests/final exam, a final paper and in-class participation will account for 75% of your final grade as follows:
      Exam 1 12
      Exam 2 12
      Exam 3 12
      Exam 4 (during final period) 12
      BiochemTract (what's that?) 15
      Assignments, drafts 7
      Instructor evaluation/class participation 3
    2. Lab-based grades will account for25% of your final grade as follows:
      Lab report 10
      Mini lab reports (3) 12
      Other lab assignments 3


1 9/5 9/7 9/10  
      Restriction digests
2 9/12 9/14 9/17  
Biochemtract paper selection due Restriction digest minilab report due in class NO CLASS PCR
3 9/19 9/21 9/24  
EXAM 1     Glycohemoglobin analysis
4 9/26 9/28 10/1  
PCR minilab report due in class   NO LAB
5 10/3 10/5 10/8  
      Glycoprotein isolation I
6 10/10 10/12 10/15  
  EXAM 2 BiochemTract draft due Glycoprotein isolation II
7 10/17 10/19 10/22  
    Carbohydrate minilab report due (in lab) Analysis of cellular lipids
8 10/24 10/26 10/29  
Biochemtract response due   EXAM 3 Isolation of detergent resistant membrane proteins I
9 10/31 11/2 11/5  
      Isolation of detergent resistant membrane proteins II
10 11/7 11/9 11/12  
    Final Lipid Lab Report Due in class NO LAB W or Th
Final Biochemtract Due at Final Exam

TENTATIVE  BIOLOGY 380 LECTURE OUTLINE  (We may cover a greater or fewer number of topics, depending upon the time available)

Topics for Lectures
Book chapters
1 Intro 1
2 Water and Buffers 2
3,4 Nucleotides 3.1-3.2, 23, 24.1-24.2, 24.4-24.5, 25.4-25.5, 27.2, 28.3
5,6 Carbohydrates 8
7,8 Membranes, lipids, and fates of cholesterol 9, 19.6-19.7
9,10 Oxidative phosphorylation and free radicals 17

General biochemistry links




Nucleic Acids