Questions on Laser Microscopy

1. How is fluorescence used to image molecules in microscopy?
2. Which persists longer: fluorescent or phosphorescent light?
3. What is confocal microscopy?
4. If a fluorescent dye absorbs light at 550 nm, what wavelength incident photons should be used in two-photon microscopy?  In three-photon microscopy?
5. What are laser tweezers?
6. What properties must objects have in order to be able to be trapped by a laser beam?
7. Describe how to measure the force a molecule exerts on an attached polystyrene bead using laser tweezers.
8. What is atomic force microscopy used for?
9. What is an optical lever?
10. Why doesn't the cantilever of an AFM damage the microscopic surface of a cell when it is imaged?